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24 chemin de borde rouge - Auzeville
CS52627
31326 Castanet-Tolosan cedex
FRANCE
Delphine Capela
Research director
SYMEVOL team
RESEARCH SUBJECTS
During her PhD, Delphine contributed to the sequencing and expert annotation of the first rhizobium genome, the model rhizobium Sinorhizobium meliloti. When she joined the CNRS in the group of Jacques Batut’s in 2002 she pursued the work on the postgenomics of S. meliloti in deciphering the transcriptome of S. meliloti during the symbiotic process and in free living conditions. Microarray was at that time the most powerful tool to study microbial transcriptomes. In 2008, Delphine switched to the experimental evolution project which aims at converting a non-symbiotic bacterium into a legume symbiont. In this project, she is managing the production of evolved populations and clones and is seeking for adaptive mutations responsible for the improvement of the symbiotic properties of experimentally evolved strains.
Key words
TRAINING
1997: Engineering school INSA Toulouse
1997: Master degree in Molecular and Cellular Biology and Genetics, Université Paul Sabatier, Toulouse
2000: Doctorate in molecular biology and microbial genomics, Université Paul Sabatier, Toulouse
2014: Habilitation à diriger des recherches, Université Paul Sabatier, Toulouse
RECENT FUNDING
ANR LIFEPATH (2023-2026)
TULIP New frontiers (2023-2024)
INRA SPE (2019-2020)
ANR REPLAY (2017-2020)
COLLABORATIONS
- Jean-Baptiste Ferdy, EDB, Toulouse. Projet FRAIB DYNAMIC (2020). Modeling the dynamics of adaptation of bacteria to endosymbiosis with legumes.
- Pierre-Marc Delaux, Cyril Libourel, Jean Keller, LRSV, Toulouse. Projet FRAIB gEXTRACT (2019). Sequening and assembly of the genome of Mimosa pudica. Analysis of the symbiotic transcriptome of Mimosa pudica .
- Edouardo da Rocha, Institut Pasteur, Paris. ANR Bioadapt SHAPE (2013-2016) et ANR Blanc REPLAY (2017-2020). Microbial evolutionary genomics. Parallels between natural and experimental evolution of rhizobia.
EDUCATION
Participation to workshops et final exams in the M2R "Microbiologie Moléculaire" (MM) and "Adaptation, Développement et Amélioration des plantes en présence de Microorganisme" (ADAM) in Toulouse.
COMMITTEE MEMBER
Member of the Scientific Advisory board of the Microbiologie, Adaptation, Pathogénie laboratory (Lyon).
SCIENTIFIC PRODUCTION
Granada Agudelo M, Ruiz B, Capela D, Remigi P. (2023) The role of microbial interactions on rhizobial fitness.Front Plant Sci. 14:1277262. doi: 10.3389/fpls.2023.1277262.
Libourel C, Keller J, Brichet L, Cazalé A-C, Carrère S, Vernié T, Couzigou J-M, Callot C, Dufau I, Cauet S, William Marande W, Tabatha Bulach T, Suin A, Masson-Boivin C, Remigi P, Delaux P-M, Capela D. (2023) Comparative phylotranscriptomics reveals ancestral and derived root nodule symbiosis programs. Nat Plants 9(7):1067-1080. doi: 10.1038/s41477-023-01441-w.
Doin de Moura GG, Mouffok S, Gaudu N, Cazalé AC, Milhes M, Bulach T, Valière S, Roche D, Ferdy JB, Masson-Boivin C, Capela D, Remigi P. (2023) A selective bottleneck during host entry drives the evolution of new legume symbionts. Mol Biol Evol 40(5):msad116. doi: 10.1093/molbev/msad116.
Doin de Moura GG, Remigi P, Masson-Boivin C, Capela D. (2020) Experimental Evolution of Legume Symbionts: What Have We Learnt? Genes (Basel). 11(3):339. doi: 10.3390/genes11030339.
Tang M, Bouchez O, Cruveiller S, Masson-Boivin C, and Capela D. (2020) Modulation of quorum sensing as an adaptation to nodule cell infection during experimental evolution of legume symbionts. mBio 11(1):e03129-19. doi: 10.1128/mBio.03129-19.
Daubech B, Poinsot V, Klonowska A, Capela D, Chaintreuil C, Moulin L, Marchetti M, and Masson-Boivin C. (2019) noeM, a new nodulation gene involved in the biosynthesis of Nod Factors with an open-chain oxidized terminal residue and in the symbiosis with Mimosa pudica. Mol Plant-Microbe Interact. 32:1635-1648. doi: 10.1094/MPMI-06-19-0168-R.
Clerissi C, Touchon M, Capela D, Tang M, Cruveiller S, Parker MA, Moulin L, Masson-Boivin C, Rocha EPC. (2018) Parallels between experimental and natural evolution of legume symbionts. Nat Commun. 9(1):2264. doi: 10.1038/s41467-018-04778-5.
Daubech B, Remigi P, Doin de Moura G, Marchetti M, Pouzet C, Auriac MC, Gokhale CS, Masson-Boivin C, Capela D. (2017) Spatio-temporal control of mutualism in legumes helps spread symbiotic nitrogen fixation. Elife. 6: e28683. doi: 10.7554/eLife.28683.
Capela D, Marchetti M, Clérissi C, Perrier A, Guetta D, Gris C, Valls M, Jauneau A, Cruveiller S, Rocha EPC, Masson-Boivin C. (2017) Recruitment of a lineage-specific virulence regulatory pathway promotes intracellular infection by a plant pathogen experimentally evolved into a legume symbiont. Mol Biol Evol. doi: 10.1093/molbev/msx165.
Marchetti M, Clerissi C, Yousfi Y, Gris C, Bouchez O, Rocha E, Cruveiller S, Jauneau A, Capela D, Masson-Boivin C. (2017) Experimental evolution of rhizobia may lead to either extra- or intracellular symbiotic adaptation depending on the selection regime. Mol Ecol. 26(7):1818-1831.
Remigi P, Capela D, Clerissi C, Tasse L, Torchet R, Bouchez O, Batut J, Cruveiller S, Rocha EP, Masson-Boivin C. (2014) Transient hypermutagenesis accelerates the evolution of legume endosymbionts following horizontal gene transfer. PLoS Biol., 12(9):e1001942.
Marchetti M, Jauneau A, Capela D, Remigi P, Gris C, Batut J, Masson-Boivin C. (2014) Shaping bacterial symbiosis with legumes by experimental evolution. Mol. Plant Microbe Interact., 27(9):956-964.
Selami N, Auriac MC, Catrice O, Capela D, Kaid-Harche M, Timmers T. (2014) Morphology and anatomy of root nodules of Retama monosperma (L.)Boiss. Plant Soil, 379:109-119.
Roux B, Rodde N, Jardinaud MF, Timmers T, Sauviac L, Cottret L, Carrère S, Sallet E, Courcelle E, Moreau S, Debellé F, Capela D, de Carvalho-Niebel F, Gouzy J, Bruand C, Gamas P. (2014) An integrated analysis of plant and bacterial gene expression in symbiotic root nodules using laser capture microdissection coupled to RNA-seq. Plant J., 77(6):817-37.
Sallet E, Roux B, Sauviac L, Jardinaud MF, Carrère S, Faraut T, de Carvalho-Niebel F, Gouzy J, Gamas P, Capela D, Bruand C, Schiex T. (2013) Next Generation Annotation of prokaryotic genomes with EuGene-P: application to Sinorhizobium meliloti 2011. DNA Res., 20(4):339-54.
Guan SH, Gris C, Cruveiller S, Pouzet C, Tasse L, Leru A, Maillard A, Médigue C, Batut J, Masson-Boivin C, Capela D. (2013) Experimental evolution of nodule intracellular infection in legume symbionts. ISME J. doi: 10.1038/ismej.2013.24.
Marchetti M, Capela D, Poincloux R, Benmeradi N, Auriac MC, Le Ru A, Maridonneau-Parini I, Batut J, Masson-Boivin C. (2013) Queuosine biosynthesis is required for Sinorhizobium meliloti-induced cytoskeletal modifications on HeLa cells and symbiosis with Medicago truncatula. PLoS One, 8(2):e56043.
Marchetti M*, Capela D*, Glew M*, Cruveiller S, Chane-Woon-Ming B, Gris C, Timmers T, Poinsot V, Gilbert LB, Heeb P, Médigue C, Batut J, Masson-Boivin C. (2010) Experimental evolution of a plant pathogen into a legume symbiont. PLoS Biol., 8(1):e1000280. *Co-premiers auteurs.
Becker A, Barnett MJ, Capela D, Dondrup M, Kamp PB, Krol E, Linke B, Rüberg S, Runte K, Schroeder BK, Weidner S, Yurgel S, Batut J, Long SR, Pühler A, Finan TM, Goesman A. (2009) A portal for rhizobial genomes: RhizoGATE integrates a S. meliloti genome annotation update with postgenome data. J. Biotechnol., 140(1-2):45-50.
Peltzer M, Roques N, Poinsot V, Aguilar OM, Batut J, Capela D. (2008) Auxotrophy accounts for nodulation defect of most Sinorhizobium meliloti mutants in the branched-chain amino acid biosynthesis pathway. Mol. Plant Microbe Interact., 21(9):1232-41.
Rossbach S, Mai DJ, Carter EL, Sauviac L, Capela D, Bruand C, de Bruijn FJ. (2008) Response of Sinorhizobium meliloti to elevated concentrations of cadmium and zinc. Appl. Environ. Microbiol., 74(13):4218-21.
Amadou C, Pascal G, Mangenot S, Glew M, Bontemps C, Capela D, Carrère S, Cruveiller S, Dossat C, Lajus A, Marchetti M, Poinsot V, Rouy Z, Servin B, Saad M, Schenowitz C, Barbe V, Batut J, Médigue C, Masson-Boivin C. (2008) Genome sequence of the β-rhizobium Cupriavidus taiwanensis and comparative genomics of rhizobia. Genome Res. 18(9):1472-83.
Capela D, Filipe C, Bobik C, Batut J, Bruand C. (2006) Sinorhizobium meliloti differentiation during symbiosis with alfalfa: a transcriptomic dissection. Mol. Plant Microbe Interact., 19(4):363-372.
Capela D, Carrère S, Batut J (2005) Transcriptome-based identification of the NodD1 regulon of Sinorhizobium meliloti. Appl. Environ. Microbiol., 71(8):4910-4913.
Maillart E, Brengel-Pesce K, Capela D, Roget A, Livache T, Canva M, Levy Y, Soussi T (2004). Versatile analysis of multiple macromolecular interactions by SPR imaging: application to p53 and DNA interaction. Oncogene, 23(32):5543-5550.
Becker A, Berges H, Krol E, Bruand C, Ruberg S, Capela D, Lauber E, Meilhoc E, Ampe F, de Bruijn FJ, Fourment J, Francez-Charlot A, Kahn D, Kuster H, Liebe C, Puhler A, Weidner S, Batut J (2004). Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions. Mol. Plant Microbe Interact., 17(3):292-303.
Djordjevic MA, Chen HC, Natera S, Van Noorden G, Menzel C, Taylor S, Renard C, Geiger O, Weiller GF, Sinorhizobium DNA Sequencing Consortium (2003). A global analysis of protein expression profiles in Sinorhizobium meliloti: discovery of new genes for nodule occupancy and stress adaptation. Mol Plant Microbe Interact, 16(6):508-24.
Barnett MJ, Fisher RF, Jones T, Komp C, Abola AP, Barloy-Hubler F, Bowser L, Capela D, Galibert F, Gouzy J, Gurjal M, Hong A, Huizar L, Hyman RW, Kahn D, Kahn ML, Kalman S, Keating DH, Palm C, Peck MC, Surzycki R, Wells DH, Yeh KC, Davis RW, Federspiel NA, Long SR (2001). Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid. Proc Natl Acad Sci U S A, 98(17):9883-8.
Capela D, Barloy-Hubler F, Gouzy J, Bothe G, Ampe F, Batut J, Boistard P, Becker A, Boutry M, Cadieu E, Dreano S, Gloux S, Godrie T, Goffeau A, Kahn D, Kiss E, Lelaure V, Masuy D, Pohl T, Portetelle D, Puhler A, Purnelle B, Ramsperger U, Renard C, Thebault P, Vandenbol M, Weidner S, Galibert F (2001). Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021. Proc Natl Acad Sci U S A, 98(17):9877-82.
Galibert F, Finan TM, Long SR, Puhler A, Abola P, Ampe F, Barloy-Hubler F, Barnett MJ, Becker A, Boistard P, Bothe G, Boutry M, Bowser L, Buhrmester J, Cadieu E, Capela D, Chain P, Cowie A, Davis RW, Dreano S, Federspiel NA, Fisher RF, Gloux S, Godrie T, Goffeau A, Golding B, Gouzy J, Gurjal M, Hernandez-Lucas I, Hong A, Huizar L, Hyman RW, Jones T, Kahn D, Kahn ML, Kalman S, Keating DH, Kiss E, Komp C, Lelaure V, Masuy D, Palm C, Peck MC, Pohl TM, Portetelle D, Purnelle B, Ramsperger U, Surzycki R, Thebault P, Vandenbol M, Vorholter FJ, Weidner S, Wells DH, Wong K, Yeh KC, Batut J (2001). The composite genome of the legume symbiont Sinorhizobium meliloti. Science, 293(5530):668-72.
Galibert F, Barloy-Hubler F, Capela D, Gouzy J (2000). Sequencing the Sinorhizobium meliloti genome. DNA Seq., 11(3-4):207-10.
Barloy-Hubler F, Capela D, Batut J, Galibert F (2000). High-resolution physical map of the pSymb megaplasmid and comparison of the three replicons of Sinorhizobium meliloti strain 1021. Curr Microbiol., 41(2):109-13.
Barloy-Hubler F, Capela D, Barnett MJ, Kalman S, Federspiel NA, Long SR, Galibert F (2000). High-resolution physical map of the Sinorhizobium meliloti 1021 pSyma megaplasmid. J. Bacteriol., 182(4):1185-9.
Capela D, Barloy-Hubler F, Gatius MT, Gouzy J, Galibert F (1999). A high-density physical map of Sinorhizobium meliloti 1021 chromosome derived from bacterial artificial chromosome library. Proc Natl Acad Sci U S A, 96(16):9357-62.
Alvinerie M, Sutra JF, Capela D, Galtier P, Fernandez-Suarez A, Horne E, O'Keeffe M (1996). Matrix solid-phase dispersion technique for the determination of moxidectin in bovine tissues. Analyst, 121(10):1469-72.
24 chemin de borde rouge - Auzeville
CS52627
31326 Castanet-Tolosan cedex
FRANCE
Key words
Anne-Claire Cazalé
Research engineer
SYMEVOL team
RESEARCH SUBJECTS
Anne-Claire has studied signalisation (reactive oxygen species production and protein kinases) in plants in response to abiotic stress during her PhD at the ISV (CNRS, Gif-sur-Yvette, France) and during her post-doc at the MPIZ (Koeln, Germany). She analyzed the heavy metal tolerance during two post-docs at the Leibniz-IPB (Halle/Saale, Germany) and at CEA Cadarache (France). She joined the LIPM in 2007 to contribute to the characterization of the type III effectors from Ralstonia solanacearum in the team of Stéphane Genin. From 2015 to 2020, she worked in the team of Claude Bruand to participate in the elucidation of the roles of nitric oxide (NO) in the Medicago truncatula-Sinorhizobium meliloti symbiotic interaction. More specifically, she studied the protein post-translational modifications induced by NO. Since 2020, Anne-Claire joined the team of Delphine Capela to analyze transcriptomic modifications in the Mimosa pudica legume in response to the progressive adaptation of experimentally evolved symbionts.
TRAINING
1999 : PhD «Sciences de la vie: Physiologie Cellulaire et Moléculaire des Plantes », University of Paris VI, France.
RECENT FUNDING
COLLABORATIONS
EDUCATION
COMMITTEE MEMBER
EDITORIAL ACTIVITY
SCIENTIFIC PRODUCTION
Libourel C, Keller J, Brichet L, Cazalé A-C, Carrère S, Vernié T, Couzigou J-M, Callot C, Dufau I, Cauet S, William Marande W, Tabatha Bulach T, Suin A, Masson-Boivin C, Remigi P, Delaux P-M, Capela D. (2023) Comparative phylotranscriptomics reveals ancestral and derived root nodule symbiosis programs. Nat Plants 9(7):1067-1080. doi: 10.1038/s41477-023-01441-w.
Doin de Moura GG, Mouffok S, Gaudu N, Cazalé AC, Milhes M, Bulach T, Valière S, Roche D, Ferdy JB, Masson-Boivin C, Capela D, Remigi P. (2023) A selective bottleneck during host entry drives the evolution of new legume symbionts. Mol Biol Evol 40(5):msad116. doi: 10.1093/molbev/msad116.
Cazalé AC, Blanquet P, Henry C, Pouzet C, Bruand C, Meilhoc E (2021) Tyrosine nitration of flagellins: a response of Sinorhizobium meliloti to nitrosative stress. AEM 87(1): e02210-20.
González-Fuente M, Carrère S, Monachello D, Marsella BG, Cazalé AC, Zischek C, Mitra RM, Rezé N, Cottret L, Mukhtar MS, Lurin C, Noël LD and Peeters N (2020) EffectorK, a comprehensive resource to mine for Ralstonia, Xanthomonas and other published effector interactors in the Arabidopsis proteome. Mol Plant Pathol 00: 1–14.
Sang Y, Wang Y, Ni H, Cazalé AC, She YM, Peeters N, Macho AP (2018), The Ralstonia solanacearum type III effector RipAY targets plant redox regulators to suppress immune responses, Mol Plant Pathol 19(1): 129-142
Lonjon F, Lohou D, Cazalé AC, Büttner D, Ribeiro BG, Péanne C, Genin S, Vailleau F. (2017), HpaB-dependent secretion of Type III effectors in the plant pathogens Ralstonia solanacearum and Xanthomonas campestris pv. Vesicatoria, Sci Rep 7(1):4879
Lonjon F, Turner M, Henry C, Rengel D, Lohou D, van de Kerkhove Q, Cazalé AC, Peeters N, Genin S, Vailleau F (2016) Comparative secretome analysis of Ralstonia solanacearum type 3 secretion-associated mutants reveals a fine control of effector delivery, essential for bacterial pathogenicity, Mol Cell Proteomics 15(2):598-613.
Poueymiro M*, Cazalé AC*, François JM, Parrou JL, Peeters N, Genin S. (2014), A Ralstonia solanacearum type III effector directs the production of the plant signal metabolite trehalose-6-phosphate, MBio 5(6) pii: e02065-14. doi: 10.1128/mBio.02065-14. (*contributed equally to this work)
Lohou D, Turner M, Lonjon F, Cazalé AC, Peeters N, Genin S, Vailleau F (2014), HpaP modulates type III effector secretion in Ralstonia solanacearum and harbours a substrate specificity switch domain essential for virulence, Mol Plant Pathol 15(6):601-14.
Peeters N, Carrère S, Anisimova M, Plener L, Cazalé AC, Genin S (2013), Repertoire, unified nomenclature and evolution of the Type III effector gene set in the Ralstonia solanacearum species complex, BMC Genomics 14:859.
CONNECTIONS
Science & Society
SCIENCE AND SOCIETY
Science & Society
HOBBY
Hobbies
OTHER AFFILIATION
Other affiliations
Nicolas Burkhardt
PhD student
SYMEVOL team
RESEARCH SUBJECTS
Analyse des convergences mutationnelles impliquées dans l’évolution adaptative de Ralstonia solanacearum à différents environnements plantes et différents modes de vie.
TRAINING
24 chemin de borde rouge - Auzeville
CS52627
31326 Castanet-Tolosan cedex
FRANCE
Key words
Master TULIP Functional Biology and Ecology (FBE)
Ph.D. student (plant-microorganism interactions, SEVAB-Université Paul Sabatier)
RECENT FUNDING
COLLABORATIONS
EDUCATION
COMMITTEE MEMBER
EDITORIAL ACTIVITY
SCIENTIFIC PRODUCTION
Publications
CONNECTIONS
Science & Society
SCIENCE AND SOCIETY
Science & Society
HOBBY
OTHER AFFILIATION
Other affiliations
RESEARCH SUBJECTS
Experimental evolution of legume symbionts
Analysis of plant transcriptomic responses to the adaptation of bacteria to endosymbiosis
TRAINING
Philippe Remigi
Researcher
SYMEVOL team
24 chemin de borde rouge - Auzeville
CS52627
31326 Castanet-Tolosan cedex
FRANCE
2011: PhD in Phytopathology, Université de Toulouse
2007: Master in Plant Biology, Université Paris-Sud, Orsay
2004: Bachelor in Biology, Ecole Normale Supérieure, Paris
RECENT FUNDING
Key words
SPE INRAE EPIMODE (2021-2022) Impact of epigenetic modifications on bacterial adaptation to a new lifestyle. Collaboration: A. Guidot (LIPME), 15 k€.
FRAIB DYNAMIC (2021-2022) Evolutionary dynamics of bacterial adaptation to symbiosis with legumes. Collaboration: J.B. Ferdy (EDB, Toulouse), 15 k€.
FRAIB gEXTRACT (2019-2020) Genomic resources for studying the evolution of plant symbioses: development of a universal protocol for high-molecular weight gDNA extraction. Partenaires : Hélène Berges (CNRGV, Toulouse) et Pierre-Marc Delaux (LRSV, Toulouse). 8 k€.
COLLABORATIONS
- Alice GUIDOT (LIPME, Toulouse)
- Jean-Baptiste FERDY (EDB, Toulouse)
- Chaitanya GOKHALE (Max Planck Institute for Evolutionary Biology, Ploen, Germany)
- Pierre-Marc Delaux (LRSV, Toulouse)
EDUCATION
Participation to workshops et final exams in the M2R "Microbiologie Moléculaire" (MM) and "Adaptation, Développement et Amélioration des plantes en présence de Microorganisme" (ADAM) in Toulouse.
COMMITTEE MEMBER
Scientific committee Labex TULIP (2019-now)
Laboratory committee LIPME (2020-now)
EDITORIAL ACTIVITY
SCIENTIFIC PRODUCTION
- Granada Agudelo M, Ruiz B, Capela D, Remigi P. (2023) The role of microbial interactions on rhizobial fitness.Front Plant Sci. 14:1277262. doi: 10.3389/fpls.2023.1277262.
- Libourel C, Keller J, Brichet L, Cazalé A-C, Carrère S, Vernié T, Couzigou J-M, Callot C, Dufau I, Cauet S, William Marande W, Tabatha Bulach T, Suin A, Masson-Boivin C, Remigi P, Delaux P-M, Capela D. (2023) Comparative phylotranscriptomics reveals ancestral and derived root nodule symbiosis programs. Nat Plants 9(7):1067-1080. doi: 10.1038/s41477-023-01441-w.
- Doin de Moura GG, Mouffok S, Gaudu N, Cazalé AC, Milhes M, Bulach T, Valière S, Roche D, Ferdy JB, Masson-Boivin C, Capela D, Remigi P. (2023) A selective bottleneck during host entry drives the evolution of new legume symbionts. Mol Biol Evol 40(5):msad116. doi: 10.1093/molbev/msad116.
- Doin de Moura G, Remigi P, Masson-Boivin C, Capela D (2020). Experimental evolution of legume symbionts: what have we learnt? Genes 11(3), 339. doi:10.3390/genes11030339.
- Remigi P, Masson-Boivin C, Rocha EPC (2019). Experimental evolution as a tool to investigate natural processes and molecular functions. Trends in Microbiology 27 (7), 623-634. doi:10.1016/j.tim.2019.02.003
- Gallie J, Bertels F, Remigi P, Ferguson GC, Nestmann, PB Rainey (2019). Repeated phenotypic evolution by different genetic routes in Pseudomonas fluorescens SBW25. Molecular Biology and Evolution 36(5), 1071-1085. doi:10.1093/molbev/msz040
- Remigi P, Ferguson GC, Rogers DW, McConnell E, De Monte S, Rainey PB (2019). Ribosome provisioning activates a bistable switch coupled to fast exit from stationary phase. Molecular Biology and Evolution 36(5), 1056-1070. doi:10.1093/molbev/msz041
- Rainey PB, Remigi P, Farr AD, Lind PA (2017) Darwin was right: where now for experimental evolution? Current Opinion in Genetics & Development 47, 102-109. doi:10.1016/j.gde.2017.09.003
- Daubech B*, Remigi P*, Doin de Moura G, Marchetti M, Pouzet C, Auriac MC, Gokhale CS, Masson-Boivin C and Capela D (2017). Spatio-temporal control of mutualism in legumes helps spreading symbiotic nitrogen fixation. eLife. 6:e28683. doi:10.7554/eLife.28683. (* denotes first co-authorship)
- Farr A, Remigi P, Rainey PB (2017). Adaptive evolution by spontaneous domain fusion and protein relocalisation. Nature Ecology and Evolution. 1: 1562-1568. doi:10.1038/ s41559-017-0283-7.
- Wang K, Remigi P, Anisimova M, Lonjon F, Kars I, Kajava A, Li CH, Cheng CP, Vailleau F, Genin S, Peeters N (2016) Functional assignment to positively selected sites in the core type III effector RipG7 from Ralstonia solanacearum. Molecular plant pathology 17 (4), 553-564. doi:10.1111/mpp.12302
- Remigi P, Zhu J, Young JPW, Masson-Boivin C (2016) Symbiosis within symbiosis: evolving nitrogen-fixing legume symbionts. Trends in microbiology 24 (1), 63-75. doi:10.1016/j.tim.2015.10.007
- Gallie J, Libby E, Bertels F, Remigi P, Jendresen CB, Ferguson GC, Desprat N, Buffing MF, Sauer U, Beaumont HJE, Martinussen J, Kilstrup M, Rainey PB (2015). Bistability in a metabolic network underpins the de novo evolution of colony switching in Pseudomonas fluorescens SBW25. PLOS Biology 13(3):e1002109. doi:10.1371/journal.pbio.1002109.
- Remigi P, Capela D, Clerissi C, Tasse L, Bouchez O, Batut J, Cruveiller S, Rocha EPC, Masson-Boivin C (2014). Transient hypermutagenesis accelerates the evolution of legume endosymbionts following horizontal gene transfer. PLOS Biology 12(9):e1001942. doi:10.1371/journal.pbio.1001942.
- Remigi P, Anisimova M, Wicker E, Genin S, Peeters N (2011). Functional divergence of the GALA family of type three effectors from the phytopathogenic bacterium Ralstonia solanacearum. New Phytologist, 192:976-987. doi: 10.1111/j.1469-8137.2011.03854.
- Reeser PW, Sutton W, Hansen EM, Remigi P, Adams GC (2011) Phytophthora species in forest streams in Oregon and Alaska. Mycologia 103 (1), 22-35. doi: 10.3852/10-013
- Remigi P, Faye A, Kane A, Deruaz M, Thioulouse J, Cissoko M, Prin Y, Galiana A, Dreyfus B, Duponnois R (2008). The exotic legume tree species Acacia holosericea alters microbial soil functionalities and the structure of the arbuscular mycorrhizal community. Applied and Environmental Microbiology 74 (5), 1485-1493. doi:10.1128/AEM.02427-07
CONNECTIONS
SCIENCE AND SOCIETY
'Sciences à Lautrec' (2019): Hosting high-school students in the laboratory to perform experiments on bacterial evolution
HOBBY
OTHER AFFILIATION
24 chemin de borde rouge - Auzeville
CS52627
31326 Castanet-Tolosan cedex
FRANCE
Saïda Mouffok
Engineer
SYMEVOL team
RESEARCH SUBJECTS
Saïda studied during her thesis at the Eukaryotic Molecular Biology Laboratory, CNRS / Toulouse University, the activity of a helicase involved in ribosome biogenesis and its interaction with proteins having specific structures (G-patch domains). She joined LIPM in 2016 to work on the Autoregulation of Root Infection (AOI) project, in the S. meliloti-Medicago symbiosis for the identification and characterization of molecular targets of the periplasmic protein involved in the AOI and whose function is unknown. Since 2020, Saïda has been involved in the experimental rhizobia evolution project. She is participating to the realization of the evolution experiment as well as the reconstruction of mutations that occurred in the experiment and the phenotyping of reconstructed mutant on plants.
TRAINING
2014 : PhD in molecular biology: Study of the structure of the helicase Prp43p and its interaction with G-patch domain proteins. “Ribosomes and Telomere” team led by Yves. Henry, Laboratory of Eukaryotic Molecular Biology, CNRS / University Toulouse III (UMR5099).
RECENT FUNDING
Key words
COLLABORATIONS
EDUCATION
COMMITTEE MEMBER
EDITORIAL ACTIVITY
SCIENTIFIC PRODUCTION
Doin de Moura GG, Mouffok S, Gaudu N, Cazalé AC, Milhes M, Bulach T, Valière S, Roche D, Ferdy JB, Masson-Boivin C, Capela D, Remigi P. (2023) A selective bottleneck during host entry drives the evolution of new legume symbionts. Mol Biol Evol 40(5):msad116. doi: 10.1093/molbev/msad116.
Sorroche F, Morales V, Mouffok S, Pichereaux C, Garnerone A. M, Zou L, Soni B, Carpene M.A, Gargaros A, Maillet F, Poinsot V, Polard P, Schiltz O, Gouch C, Batut J.2020.The ex planta signal activity of a Medicago ribosomal uL2 protein suggests a moonlighting role in controlling secondary rhizobial infection.PLos ONE D-20-05716R2.
Saïda Mouffok, Régine Capeyrou, Kamila Belhabich-Baumas, Clément Joret, Anthony K. Henras, Odile Humbert, Yves Henry. “The G-patch activators Pfa1 and PINX1 exhibit different modes of interaction with the Prp43 RNA helicase”. RNA Biology, 510-522, 2020.
Hélène Walbott, Saïda Mouffok, Régine Capeyrou, Simon Lebaron, Hermann van Tilbeurgh, Yves Henry and Nicolas Leulliot. “Prp43p contains a Hel308-like helicase structural architecture with a DEAH helicase specific regulatory domain”. EMBO J., 29, 2194-2204, 2010. (Co-premier Auteur).
Yan-Ling Chen, Régine Capeyrou, Odile Humbert, Saïda Mouffok, Simon Lebaron, Anthony K. Henras and Yves Henry. “The telomerase inhibitor Gno1p/PINX1 is an activator of the helicase Prp43p required for the synthesis of the large ribosomal subunit”. Nucleic Acids Res, May 2014.
CONNECTIONS
SCIENCE AND SOCIETY
HOBBY
OTHER AFFILIATION
24 chemin de borde rouge - Auzeville
CS52627
31326 Castanet-Tolosan cedex
FRANCE
Bryan Ruiz
Post-doctorate
SYMEVOL team
RESEARCH SUBJECTS
Functional integration of horizontally-transferred genes into bacterial genomes: nitrogen-fixing symbiosis in beta-rhizobia as a case study.
TRAINING
2022: PhD in Agrobiosciences and Ecology. Université Toulouse III.
Synthèse et rôle de l’oxyde nitrique d’origine bactérienne dans la symbiose fixatrice d’azote Ensifer meliloti-Medicago truncatula (LIPME).
RECENT FUNDING
Key words
COLLABORATIONS
EDUCATION
COMMITTEE MEMBER
EDITORIAL ACTIVITY
SCIENTIFIC PRODUCTION
Granada Agudelo M, Ruiz B, Capela D, Remigi P. The role of microbial interactions on rhizobial fitness. Front Plant Sci. 2023 Oct 9;14:1277262. doi: 10.3389/fpls.2023.1277262. PMID: 37877089; PMCID: PMC10591227.
Sauviac L, Rémy A, Huault E, Dalmasso M, Kazmierczak T, Jardinaud MF, Legrand L, Moreau C, Ruiz B, Cazalé AC, Valière S, Gourion B, Dupont L, Gruber V, Boncompagni E, Meilhoc E, Frendo P, Frugier F, Bruand C. A dual legume-rhizobium transcriptome of symbiotic nodule senescence reveals coordinated plant and bacterial responses. Plant Cell Environ. 2022 Oct;45(10):3100-3121. doi: 10.1111/pce.14389.
Ruiz B, Sauviac L, Brouquisse R, Bruand C, Meilhoc E. Role of nitric oxide of bacterial origin in the Medicago truncatula-Sinorhizobium meliloti Symbiosis. Mol Plant Microbe Interact. 2022 Oct;35(10):887-892. doi: 10.1094/MPMI-05-22-0118-SC.
Ruiz B, Frostegård Å, Bruand C, Meilhoc E. Rhizobia: highways to NO. Biochem Soc Trans. 2021 Feb 26;49(1):495-505. doi: 10.1042/BST20200989.
Ruiz B, Le Scornet A, Sauviac L, Rémy A, Bruand C, Meilhoc E. The nitrate assimilatory pathway in Sinorhizobium meliloti: contribution to NO production. Front Microbiol. 2019 Jul 3;10:1526. doi: 10.3389/fmicb.2019.01526.
CONNECTIONS
SCIENCE AND SOCIETY
HOBBY
OTHER AFFILIATION
24 chemin de borde rouge - Auzeville
CS52627
31326 Castanet-Tolosan cedex
FRANCE
Marvin Navarro
PhD student
SYMEVOL team
RESEARCH SUBJECTS
Identification of functions required for the emergence of new plant symbionts, mutualistic or pathogenic.
TRAINING
2022: Master
RECENT FUNDING
Key words
COLLABORATIONS
EDUCATION
COMMITTEE MEMBER
EDITORIAL ACTIVITY
SCIENTIFIC PRODUCTION
Publications
CONNECTIONS
SCIENCE AND SOCIETY
HOBBY
OTHER AFFILIATION
24 chemin de borde rouge - Auzeville
CS52627
31326 Castanet-Tolosan cedex
FRANCE
Marvin Navarro
PhD student
SYMEVOL team
RESEARCH SUBJECTS
Identification of functions required for the emergence of new plant symbionts, mutualistic or pathogenic.
TRAINING
2022: Master
RECENT FUNDING
Key words
COLLABORATIONS
EDUCATION
COMMITTEE MEMBER
EDITORIAL ACTIVITY
SCIENTIFIC PRODUCTION
Publications
CONNECTIONS
SCIENCE AND SOCIETY
HOBBY
OTHER AFFILIATION
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